英语释义

• a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes

片语

Endonuclease G脱氧核糖核酸酶BamHI

encoded endonuclease内含子编码内切核酸酶

endonuclease site酶切位点

spleen endonuclease脾脏核酸内切酶

restriction endonuclease限制酶，限制性内切核酸酶

restiction endonuclease限制性核酸内切酶

endonuclease analysis内切酶分析

AP endonuclease脱嘌呤嘧啶内切核酸酶

Endonuclease digestion内切酶酶切技术

英汉例句

• the recombinant plasmid pet- 11d-ospc was identified with restriction endonuclease analysis and sequencing.

  采用酶切分析及序列测定等方法鑒定重组质粒的正确性。

• in this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.

  本研究利用多种组合的酶切分析，绘制了较为详细的酶切图谱。

• results:the constructed recombinant plasmid contained the sequence of tso45-4b gene that was identified by endonuclease digestion and sequence analysis.

  结果经酶切和测序鑒定表明所构建的重组表达质粒中含有tso45-4b基因。

• these results suggest: (1)genomic dna methylation-sensitive endonuclease analysis does not reflect the overall status of total dna;

  这些结果提示：（1）用常规的酶解电泳法所分析的dna甲基化结果并不能反映整个基因组dna甲基化的水平。

• janpanese encephalitis virus; recombinant plasmid; dna sequencing; restriction endonuclease analysis.

  流行性乙型脑炎病毒；重组质粒；dna测序；限制性内切酶分析。

• the overlap extension mediated by restriction endonuclease to obtain the full length gene was established.

  建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。

• methods pcr and dna sequencing were performed to study the ar gene mutation; mbo i restriction endonuclease was used to detect existence of the mutation in normal controls;

  并对发现突变的基因进行分析。方法应用pcr扩增、dna序列测定等技术分析所有ar基因外显子及其邻近dna序列片段；

• result restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of oscp gene can be successful.

  结果酶切及测序显示对oscp基因有义突变部分的纠正获得成功。

• cp4-epsps gene was amplified from 13 samples, but all of them were proved to be false positives in restriction endonuclease digestion analysis of pcr products.

  有13个样品在cp4-epsps基因检测中虽扩增出类似阳性对照的条带，但对pcr产物的进一步酶切鑒定表明，所有扩增结果为假阳性。

• result the recombinant was correctly constructed and restriction endonuclease analysis and pcr amplification.

  结果 经酶切鑒定及基因测序证实重组三突变型穿梭质粒构建成功。

• results pcr, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of ymdd, yvdd and yidd were constructed successfully.

  结果经pcr方法、酶切鑒定及直接测序鑒定出ymdd、yvdd和yidd质粒构建成功，且该方法具有较好的敏感性和特异性。

• the endonuclease digestion map of pwta was consistent with the fragment length in anticipation.

  内切酶酶切片段与预期片段长度一致；

• after a restriction endonuclease cleavage, two expected smaller fragments were observed.

  经限制性内切酶反应，获得两个预期大小的片段。

• finally, we could evaluate plasmids cdna extracted with mono-restriction endonuclease enzyme and the agar gel electrophoresis.

  最后用限制性内切酶单酶切及琼脂凝胶电泳进行鑒定。

• pcr products were digested with endonuclease and sequenced.

  pcr产物经限制性内切酶消化、序列测定及分析。

• methods dnas were extracted from the white blood cells of people in hainan by salt-out method. polymerase chain reaction and restriction endonuclease was used to determine the 4533g/a polymorphism.

  方法用盐提取法提取人群中白细胞的dna，以聚合酶链反应、限制性内切核酸酶检测-4533g/a多态性。

• methods:dna sequencing and restriction endonuclease reaction was used.

  方法：采用dna测序及限制性酶切反应方法。

• the products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining.

  用8%非变性聚丙烯酰胺凝胶电泳将酶切产物分离，并用银染法显色。

• secondly, the preamplified dna fragments were digested by a restriction endonuclease to form sticky ends, which were then ligated to a designed dna adapter by ligase.

  然后用限制性内切酶将其消化成短片段，在连接酶的作用下与设计的dna适配器相连；

• the products of endonuclease cutting were detected by 8 percent non-denatured polyacrylamide gel electrophoresis and showing in silver staining protocol.

  用8%非变性聚丙烯酰胺凝胶电泳将酶切产物分离，并用银染法显色。