on the basis of inverse pcr, we have founded a ipcr technique to clone foreign gene's flanking sequence in transgenic rice, which is suitable to treat mass materials.
以反向pcr(ipcr)为基础建立了适合于处理大量材料的克隆转基因水稻中外源基因旁侧序列的技术体系。
the result showed that this ipcr technique is quick, convenient and stable for flanking sequence cloning.
实验结果表明这个方法具有快速、稳定和高效的优点。
but the flanking sequence of the t331 and t415 were located at non-coding region between genes which are predicted by using software fgenesh 1.1.
突变体t331的旁邻序列和突变体t415的旁邻序列都位于fgenesh1.1预测的两个基因之间的非编码区。
in modern research of molecular biology, we usually need to analyse or clone these flanking sequence in given sites, so as to study gene expression and control.
在现代分子生物学技术研究中,常常需要对已知位点的侧翼序列进行分析或克隆,以研究基因的遗传表达调控。