methods for separation and quantitation of the adenosine polyphosphate in rabbit brain tissue by isocratic ion-pair reversed-phase high-performance liquid chromatography (hplc) are described.
采用反相离子对高效液相色谱法测定兔脑提取液中的磷酸腺苷。
then, i use another 10-min gradient to reequilibrate from the final conditions back to the initial conditions, followed by 8 min of isocratic hold.
然后,我用另外10分钟的梯度把最终的条件重新平衡到初始的条件,接着保持8分钟等度洗脱。
secondly, type of elution was considered and type of isocratic elution was attempted firstly.
第二是洗脱方式,可先用等度洗脱方式进行尝试。
both gradient and isocratic elution are described for rapid selection of starting condition for modifier optimization in reversed-phase hplc.
本文从梯度和等浓度两方面讨论了反相色谱流动相组分最优化过程中初始条件的迅速选择。
the reactions were carried out in an isocratic reactor and an oil bath fixed bed reactor, which are designed by us.
反应是在实验室自行设计的无梯度反应器和油浴固定床反应器中进行。
this isocratic system consists of a high pressure solvent delivery pump, an uv/vis detector, a manual injection valve, and a solvent tray, a column and a chromatography workstation.