isoelectric focusing
[生化] 等电点聚焦
2025-10-31 22:22 浏览次数 9
[生化] 等电点聚焦
1. an electrophoretic technique for separating proteins by causing them to migrate under the influence of an electric field through a medium (such as a gel) having a pH gradient to locations with pH values corresponding to their isoelectric points
2. an electrophoretic technique for separating proteins by causing them to migrate under the influence of an electric field through a medium (as a gel) having a pH gradient to locations with pH values corresponding to their isoelectric points
its molecular weight is 64,000 and isoelectric point around ph 4.2 as determined by sds-page and isoelectric focusing analysis respectively.
sds聚丙烯酰胺凝胶电泳测定分子量为64,000。等电聚焦电泳测得等电点在4.2左右。
methods:in csf and serum from 398 patients with neurological diseases , 51 of whom had ms, ocb were detected using isoelectric focusing with immunofixation.
方法:收集3 98例神经系统疾患的脑脊液和血清标本,其中5 1例ms患者,用等电聚焦加免疫标记的方法进行ocb的检测。
capillary isoelectric focusing (cief) is a more rapid technique for separating proteins that the traditional gel-based technique.
和传统的凝胶技术相比,毛细管等电聚焦(cief)是一种蛋白质的快速分离技术。
isoelectric focusing may be used as another indication.
等电聚焦可以用为另一指标。
after being concentrated and desalted, the samples were separated by isoelectric focusing on first dimension and sds electrophoresis on second dimension.
样品浓缩 除盐后,进行一维等电聚焦分离和二维sds电泳分离提取人肾组织磷酸化蛋白。
esterase isozyme upon isoelectric focusing eletrophores demonstrated that the high levels of total esterase activity in abm r was due to the increased activities of e7, e13 and e15 isozymes.
从酯酶同工酶等电聚焦电泳得出,abm-r种群的e7、e13和e15同工酶活力显着提高是导致abm-r种群酯酶活力提高的主要原因。
the keratin compositions of the human and animal hairs belonging to 6 orders, 15 families and 20 species was studied by polyacrylamide gel isoelectric focusing (pagief).
本文用聚丙烯酰胺等电聚焦(pagief)对来自6个目、15个科、20种动物毛发角蛋白组分进行了研究。
inheritance of pgi isozymes in chestnut species was analyzed using isoelectric focusing on thin layer polyacrylamide slab gels and single tree progeny method.
采用超薄聚丙烯酰胺平板凝胶等电聚焦电泳和单株后代法,分析了栗属种的pgi同工酶的遗传。
result of isoelectric focusing electrophoresis shows that copper and zinc superoxide dismutase from peking duck erythrocytes contains five major active components with isoelectric points at ph5.
等电聚焦表明,北京鸭红细胞铜锌超氧化物歧化酶由等电点分别为5。
isoelectric focusing of stfc in thin-layer polyacrylamide gel obtained only one band as shown by coomassie blue staining.
在等电点聚焦分析中该成分为一条考马斯亮蓝染色带。
in the present work the new technique of isoelectric focusing with some modifications is used in the determination of the isoelectric points of parent and modified gelatins.
我们采用等电聚焦的新技术,经过适当的改变,成功地测定了改性明胶的等电点及其分布。
by using rapd technique and isoelectric focusing electrophoresis method, genomic dna and protein from guangdong strain and fujian strain of echinochasmus fujianensis were analysed respectively.
采用rapd技术和等电聚焦电泳方法 ,对福建棘隙吸虫福建株和广东株虫体进行基因组dna多态性分析和蛋白质等电点测定 。
the component and isoelectric point of all parts of the sample were deter- mined by capillary isoelectric focusing (cief).
经毛细管等电聚焦电泳(cief)的方法测定其组成及各组分的等电点。
methods: saliva was collected from 33 healthy and 26 periodontitis individuals respectively, and analysed with isoelectric focusing in polyacrylamide gels.
方法:采集33例正常人,26例牙周炎患者全唾液,运用聚丙烯酰胺凝胶等电聚焦方法观察其蛋白质等电点分布。
isoelectric focusing a technique used in electrophoresis to separate amphoteric molecules (able to combine with either acids or bases).
是一种利用电泳技术分离两性分子(能与酸或堿结合)的技术。