This is a reliable method to select the hybridoma cells for McAb of IBDV.
是研究筛选抗ibd V单抗杂交瘤细胞的可靠方法。
A double antibody sandwich ELISA for RV antigen was developed using McAb 4c2 as capture antibody and rabbit PcAb as detection antibody.
以单克隆抗体4c2作为捕捉抗体,兔多克隆抗体作为检测抗体,建立了ELISA双抗体夹心法,检测RV抗原。
Along with the addition of ammonia, the concentration of McAb in culture supernatant was decreased, however, the specific production rate of it was increased.
氨的添加降低了培养上清中的单抗浓度,但提高了单抗的比生产速率。
On SDS-polyacrylamide gel electrophoresis (SDS-PAGE)the eluate from the column with the McAb coupled showed a single band, corresponding to the elution peak.
聚丙烯酰胺凝胶电泳显示单抗偶联柱层析洗脱物仅有单一区带与洗脱峰对应;
The examination upon a lazer scan confocal microscope revealed the expression of the differentiation antigen recognized by McAb 6A8 both on the membrane and in the cytoplasm of 3D5 cell.
激光扫描共聚焦显微镜观察显示,单抗6A8识别的分化抗原见于3D5细胞浆和胞膜。
The titer of McAb of Mice hydroperitonia reached to 1:12800;
小鼠腹水单克隆抗体的最高稀释度达1:12800以上;
Conclusion the human McAb can be used for the prevention and treatment of tetanus infection.
结论人源单抗可用于破伤风感染的预防治疗。
The recombinant protein could specially react with B7 2 McAb and could stimulate T cell proliferation combined with anti CD3 antibody.
在抗人CD3单抗的协同下,B72胞外区重组蛋白能有效地促进外周血单核细胞的增殖。
Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.
方法应用淋巴细胞杂交瘤技术,制备单克隆抗体,并对其进行鑒定。
Objective To prepare the McAb against DNA and use for the development of a specific, sensitive and simple method for the determination of residual exogenous DNA.
目的制备抗d NA单克隆抗体,用以建立特异、灵敏、简便的外源性DNA残留量测定方法。
Objective to investigate the relationship between PF18 3 McAb recognized molecule and thymocyte activation induced apoptosis.
目的研究PF 183单抗识别分子和胸腺细胞活化及凋亡的关系。
The 72 45 McAb of tumor associated glycoprotein (TAG) 72 antigen and two kinds of lectins PNA and DBA were expressed in large bowel carcinoma of different histologic patterns and transitional mucosa.
应用肿瘤相关糖蛋白72抗原(TAG - 72)的单抗72- 45及两种凝集素pna和dba对不同组织分型的大肠癌及癌旁组织进行了研究。
Conclusion: the cell line 5e10 could secret the purpose McAb, AFB_1 immunoaffinity column prepared by the McAb needs to be further optimized.
结论:杂交瘤细胞株5e10能稳定的分泌目的单克隆抗体,利用该抗体初步研制的AFB_1免疫亲和柱的性能仍需进一步优化。
A one step dot ELISA using this specific McAb and a 2nd antibody was used for detection of circulating schistosome antigen in the urine of infected rabbits.
用单抗二抗一步法酶联免疫试验检测感染家兔中的日本血吸虫循环抗原。