method:the mutation and deletion of exon5, exon6 and exon8 of pten gene were detected by pcr, gene clone and sequencing in mucoepidermoid carcinoma of parotid gland and normal gland tissue.
方法:应用聚合酶链反应(pcr)及克隆、基因测序的方法来分析腮腺粘液表皮样癌及正常腺体组织中pten基因第5、6、8外显子有无突变及缺失。