it was found that these three compounds can induce the differentiation of hl-60 cells in vitro, judged by cell growth inhibition, morphologic observation and nbt reduction ability.
结果表明,在适当的用药浓度下,从细胞生长抑制情况、形态学观察及nbt还原能力测定判断,三种药物对hl-60细胞有明显的诱导分化作用。
methods: in vitro cell culture, mtt titration, nbt reduction and phagocytosis determination were employed to study the effects of jlc on hl-60 cells.
方法:利用体外细胞培养、mtt、nbt染色及吞噬功能测定等方法,观察在不同浓度金龙胶囊作用下人早幼粒白血病细胞系(hl-60)的细胞形态和功能的变化。
nbt reduction test showed functional maturity of the differentiated hl-60 cells.
四唑氮蓝)还原试验显示其功能亦渐超成熟。
differentiation rate of the dmsoinduced hl60 cells was tested by nbt reduction assay.
nbt还原比色法检测细胞的诱导分化能力。
methods: mtt assay to test cytotoxicity; cell staining and nbt reduction to test cell differentiation.
方法:mtt法测定细胞增殖,细胞染色和nbt还原试验测定细胞分化。