the capture of the labeled antibody–analyte complex for signal generation can be achieved with the antibody immobilized in a defined area of nitrocellulose membrane as mentioned.
对产生信号的标记抗体-抗原复合物的捕获,是用抗体固定在已经提到的硝酸纤维素的特定区域内来达到的。
methods the solid state carrier with nitrocellulose membrane substituting for polystyrene plate among the convention elisa, the other step was operated according to elisas method.
方法以硝酸纤维素膜代替常规elisa中的固相载体聚苯乙烯板,其余步骤按elisa方法操作。
since the flow rate in the nitrocellulose membrane is much slower than that through the glass pad, the material transfer to the upper compartment is the rate-limiting step.