the polymorphism fragments was cloned and sequenced. the result revealed a single nucleotide substitution of c→g at position 1006 for haeiii-rflp.
对此多态性片段进行克隆测序分析,结果表明此酶切位点是由于1006位的堿基c→g的突变引起。
influence of neighboring base composition on nucleotide substitution bias was observed in the sequence of nuclear pseudogenes and chloroplast non-coding and coding sequences.