objectived: to investigate in vitro and vivo biological characteristics of primary culture of human primordial germ cells (pgc) on fibroblast feeder layer of human endometrium.
目的:观察接种于人子宫内膜成纤维细胞饲养层上原代培养的人原始生殖细胞在体内外的生物学特性。
it was found the number of primordial germ cells in the blood and gonads decreased significantly, the histological structure of some organs in quail embryos had different changes.
本实验利用回转器旋转模拟微重力的生物效应,以鹌鹑胚胎作为实验材料,研究了鹌鹑胚胎生殖腺内原始生殖细胞的数目及内脏器官的组织学变化。
when cultured on feeder cells in the presence of many kinds of cytokines, primordial germ cells can proliferate continually and maintain undifferentiated state.
在饲养细胞层和多种生长因子的共同作用下,可保持原生殖细胞在体外不断增殖而不分化,最终建立eg细胞系。
conclusion? primordial germ cells and female and male germ cells in the in vivo normal development process or cultured in vitro would die.
在体正常发育过程和体外培养的原始生殖细胞和雌雄性生殖细胞都会发生凋亡。
here, we show that removing the translational repressor nanos from either gscs or their precursors, the primordial germ cells (pgcs), causes both cell types to differentiate into germline cysts.
作者表明从gsc或者其前体原始生殖系细胞(pgcs)去除翻译抑制物nanos将使两种细胞分化成生殖系包囊。
primordial germ cells came from pole cells and moved in two masses to the laterodorsal abdomen of embryo, and then were enveloped by mesoderm to form germinal ridge.
原始生殖细胞来自极细胞,分两团移至胚胎腹部侧背面,被中胚层细胞包裹,形成生殖脊。
mammalian primordial germ cells form and migrate to the gonad during embryonic development.
在胚胎发育时期,原始生殖细胞形成并迁移至生殖腺,形成种系囊。