英语释义

• any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology

片语

restriction endonuclease限制酶，限制性内切核酸酶

restriction enzyme fingerprinting限制酶指纹分析

restriction enzyme digest酶切分析

restriction endonuclease enzyme analysis method限制性内切酶分析法

restriction E enzyme限制性内切酶

hind restriction enzymehindⅢ酶

restriction enzyme PCR限制酶PCR

restriction enzyme site酶切位点

restriction enzyme pattern analysis限制性酶切分析

restriction enzyme cleavage限制性酶切

英汉例句

• note: the underlined are restriction enzyme cutting sites.

  注：下划线部分为限制性酶切位点。

• restriction enzyme site analysis was used to confirm the mutation.

  突变位点经限制性内切酶分析证实。

• both genes were correctly cloned and identified by pcr, restriction enzyme digestion and sequencing.

  经pcr鑒定、酶切鑒定和测序说明所克隆的两种基因是正确的。

• methods: 20 cases of mds patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(pcr) technique.

  方法：用甲基化敏感的限制性核酸内切酶消化，结合聚合酶链反应（pcr）技术。

• compared with age, ce is more outstanding in resolution and detection time, and it can be applied as a more effective means for dna restriction enzyme pattern analysis.

  结果表明，ce的分离效能明显高于age，是研究dna限制性内切酶谱的更有效的检测手段。

• restriction enzyme digestion and at clone were employed to rapidly screen out the recons of pa segments for the preparation of the gene chip probes.

  利用酶切、at克隆方法快速分析筛选出保护性抗原基因片段的重组子，从而制备成芯片探针。

• results restriction enzyme analysis and dna sequence analysis showed that pten gene was cloned and the eukaryotic expression vector was constructed successfully.

  结果酶切和测序证实pten基因克隆和真核表达载体构建成功。

• restriction enzyme mapping and sds-page protein analysis were done on four transformants.

  还进行了限制酶酶切分析和蛋白质sds-page分析。

• to improve the discrimination power of aflp for peanut cultivars of similar origin, different restriction enzyme combinations and more primer pairs may be needed.

  利用aflp技术要揭示相似来源的花生品种的遗传变异性，可能需要采用不同的限制酶组合、并采用更多的引物对。

• plasmid dnas were prepared by alkali lysis and purified with polyethylene glycol 8000. restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment.

  限制性酶切图谱分析证实这两个基因的结构是完整的，符合转基因实验要求。

• methods:polymerase chain reaction(pcr) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 cmt1 patients and 30 normal controls.

  方法：应用聚合酶链反应（pcr）-双酶切法，对23例cmt1患者和30例正常人进行基因特异性连接片段的检测。

• subcloning and comparative restriction enzyme analysis were carried out with the replication origin from tne integrated f' plasmid and that from the autonomous f plasmid.

  对这一复制起点和来自自主状态的f质粒的复制起点进行了亚克隆，并作限制性内切酶酶切分析比较，没有发现两者在结构上有差异。

• methods isolates were identified as acinetobacter calcoaceticus by using antibiotic susceptibility test, plasmid profiles, restriction enzyme fingerprinting assay and plasmid elimination method.

  方法对我院不动桿菌的感染进行调查，采用药敏试验、质粒抽提和限制性核酸内切酶分析法、质粒消除试验。

• restriction enzyme map simulated by pdraw32 software is consistent basically with the previous report.

  模拟电子酶切图谱与先前的报道基本一致；

• when genome transplantations were performed using the restriction enzyme minus recipient cells, all the genome transplantations worked regardless of if the dna was methylated or not.

  结果显示不论dna甲基化与否，所有使用受体细胞限制性内切酶的基因组移植都获得成功。

• this is the website of the restriction enzyme database.

  这是限制酶数据库的网站。

• results:after being identified by pcr, restriction enzyme digestion and sequencing, the adeno-integrase hybrid system was successfully constructed.

  结果：经pcr，酶切及测序方法鑒定，该腺病毒-整合酶嵌合系统构建成功。

• results the constructed vectors of ebo-wt and ebo-g87 were identified by restriction enzyme digestion and nucleotide sequencing.

  结果构建的ebog87和ebowt重组载体经内切酶双酶切鑒定及核苷酸序列测定证实。

• conclusion:because of the speediness, simpleness and good specificity, the pcr combined with restriction enzyme digestion can be used as a primary screening in the gene diagnosis of cmt1a.

  结论：由于pcr-双酶切方法快速、简单、易操作，且特异性好，可作为cmt1a基因诊断的一种初筛方法。

• by restriction enzyme identification, pcr and sequence analysis, the recombinant plasmid of the ha1 was successfully constructed.

  结果经双酶切、pcr及测序鑒定证实血凝素基因ha1区的真核表达载体构建成功。

• positive clones of f gene were identified by pcr and restriction enzyme digestion and then sequenced.

  经质粒pcr及酶切鑒定后，对阳性克隆进行测序。

• methods allele-specific restriction enzyme was used to analyze patient, her mother and brother and 40 healthy volunteers.

  方法用限制性内切酶分析患者和其直系亲属以及40例正常人等位基因；