western blot
蛋白质印迹
2025-07-21 17:48 浏览次数 9
蛋白质印迹
1. a blot consisting of a sheet of nitrocellulose or nylon that contains spots of protein for identification by a suitable molecular probe and is used especially for the detection of antibodies
2. a blot consisting of a sheet of cellulose nitrate or nylon that contains spots of protein for identification by a suitable molecular probe and is used especially for the detection of antibodies — compare northern blot, southern blot
Western Blot Transferring buffer蛋白杂交转膜液
análise western blot蛋白质印迹解折
western dot blot斑点免疫印迹技术
Western Blot Wash BufferWB洗涤液
FAR WESTERN BLOT远西方杂交
western western blot生物
Western- blot蛋白质印迹
Western blot ELISA蛋白质免疫印迹酶联免疫吸附法
the expression of the above three ha dna vaccines was verified in 293t cells by western blot analysis.
用这3种重组质粒分别转染293t细胞,蛋白表达经蛋白质印迹检测得到确认。
the phosphorylation of myosin binding subunit(mbs) of myosin phosphatase-a substrate of rho kinase was detected by western blot as a marker of functional activation of the kinase.
免疫印迹法检测其底物肌球蛋白磷酸酶的磷酸化状态,作为该激酶功能活化的标志。
methods:51 cases of early syphilis were summarized, rpr, fta abs, western blot and pcr techniques were compared in the diagnosis.
方法:总结51例早期梅毒的临床表现,对rpr、fta-abs、蛋白印迹法以及pcr等多种诊断方法进行评价。
western blot assay shows the purified antibody have higher specificity.
实验表明纯化后的抗体具有较高特异性。
the content of heat shock protein 70 in myocardium was detected with western blot method.
采用蛋白印迹法测定心肌细胞中热休克蛋白70含量。
common technologies used for hcp analysis include elisa (enzyme-linked immunosorbent assay), one-dimensional or two-dimensional gel electrophoresis, western blot and mass spectrometry.
常见的用于宿主细胞蛋白的分析的技术包括酶联免疫吸附法(酶联免疫吸附试验),一维或二维凝胶电泳,免疫印迹和质谱分析。
western blot analysis of cos cell extracts, untransfected or transiently transfected with a construct expressing dkk1, using dkk1 antibody.
使用dkk1抗体对未转染或已转染dkk1表达质粒的cos细胞提取物进行western blot分析。
similar results of both flow cytometry and western blot showed a high coincidence of the two methods. flow cytometry, however, was more simple, rapid, reliable and suitable for study of large samples.
两种方法所得结果具有高度一致性,但流式细胞术操作简单、省时,可测样本量大,结果可靠,因此更适合于大样本快速研究。
western blot with anti-human il-2 mcab showed that the expressed product was designed chimeric protein.
以鼠抗人il-2单克隆抗体经免疫印迹验证,该表达蛋白为所设计的嵌合蛋白。
methods: the expression of p16 protein was examined by western blot in 6 normal postmortem pituitary specimens and 40 pituitary adenoma specimens which were confirmed by pathology.
方法:取尸体解剖正常垂体标本6例,临床病理证实的垂体腺瘤标本40例,采用蛋白杂交技术检测p16蛋白的表达;
western blot analysis of extracts of various cell lines using tace antibody.
使用tace抗体对多种细胞提取物进行western blot分析。
methods mmp-9 and timp proteins were examined by using zymographic analysis and western blot in 41 cases with escc.
方法免疫组化法分别检测41例食管鳞癌患者的癌及相应正常组织中mmp-9和timp的表达变化。
objective to develope a western blot of human plasma factor v (fv) and conduct immunoblot analyses on riasma fv from a family with hereditary fv deficiency.
目的建立人血浆因子v(fv)蛋白免疫印迹分析,对遗传性fv缺乏癥家系成员血浆fv含量进行分析。
the western blot indicated that the expressed kininogenase had the antigenicity to human serum kininogenase.
蛋白质免疫印迹实验表明,重组蛋白具有激肽原酶的抗原性。
results the rsv g glycoprotein was expressed by the recombinant vaccinia virus, which was determined by western blot with anti-g protein specific monoclonal antibody.
结果rsv地方株g蛋白在痘苗病毒中获得较好的表达,表达产物的糖基化程度较高,且该重组病毒免疫家兔可诱发特异性抗体产生。
method:using western blot method to measure hsp of hypothalamus in febrile and hypothermal rats.
方法:取高、低体温大鼠下丘脑组织,应用蛋白印迹法测定热休克蛋白含量。
pcr and western blot were used to select the transgenic tobacco plants. elisa was used to evaluate the expression level while colony forming assay was used to test its biological activity.
蛋白印迹实验鑒定转基因植株,elisa方法测定重组蛋白表达水平,集落形成实验测定重组蛋白活性。
western blot analysis of the gst-tm fusion protein using sera from subjects with crustacean allergy confirmed that tm is the major allergen of chinese mitten crab.
通过与甲壳类过敏患者血清的免疫印迹反应,证实融合表达的原肌球蛋白具有过敏原性,表明原肌球蛋白是蟹类的主要过敏原之一。
by ifa, elisa and western blot experiments, it was proved all the recombinant baculoviruses containing so. 7 segment could express biologically active proteins in insect cells.
间接免疫荧光、elisa和免疫印迹检测证明,含so.7片段的三种重组桿状病毒均在昆虫细胞中表达出相应的活性蛋白。
the mcabs were analysed and characterized with eia and western blot et al. and were compared with imported polyclonal antibodies (poabs).
通过酶免疫测定及免疫印迹等法对单克隆抗体进行分析及鑒定,并通过免疫组化方法与进口多克隆抗体进行比较。