Objective In order to supply an effective reference of early injury time estimation and explore the time limit of detection of EDA\EDB mRNA in human skin samples,the expression of alternative splic-ing segment of fibronectin-EDA\EDB in incised wound of human skin were studied.
The expression of FN-EDA\EDB in human skin incised wound showed a gradually increased tendency in early injury time(within3h).
在损伤早期(≤3h)FNEDA、EDB的表达随时间的延长而呈现一种不断上升的趋势;
Methods Using in situ hybridization with DIG-labeled anti-sense RNA probe,the expression of FN EDA\EDB domain was detected in human skin incised wound at the early stage of injury(from30min to3h).