objective: to modify the monolayer culture method of human fetal islet cells.
目的:改良消化培养人胎胰岛细胞培养方法。
methods chick embryo joint chondrocytes digested and isolated by enzyme were grown monolayer culture in vitro. chondrocyte culture media was collected.
方法用酶消化、分离鸡胚关节软骨细胞,体外单层培养,收集细胞培养液。
method:primary cells of human degenerative af were obtained through in vitro monolayer culture combined with enzyme digestion method and tissue pieces culture method.
方法结合酶消化法和组织块培养法,体外单层培养人退变纤维环原代细胞。
the results showed that rat hepatocytes in gel-entrapment culture and monolayer culture were both susceptible to sodium salicylate toxicity.
第三部分讨论了凝胶包埋培养大鼠肝细胞用于药物肝毒性研究的可行性。
however, traditional monolayer culture of hepatocytes cannot maintain liver-specific functions and cytochrome p450 activities for a long time, which limited its application in drug testing in vitro.
传统的单层贴壁培养不能长期保持肝细胞功能和细胞色素酶活性,这些限制了它在药物研究中的应用。
furthermore, corneal engineering has developed from monolayer culture to threedimensional culture, and the engineered tissue becomes more close to the structure and function of normal cornea.