eukaryotic expression
真核表达
2025-11-23 20:45 浏览次数 9
真核表达
eukaryotic cell expression真核细胞表达
eukaryotic plasmid expression victors真核表达载体
Eukaryotic Gene Expression真核细胞基因表达
eukaryotic cell expression recombinant真核表达重组体
eukaryotic c expression真核表达
eukaryotic cells expression plasmid真核细胞表达质粒
eukaryotic secretory expression真核分泌表达
eukaryotic cells expression真核细胞表达
eukaryotic cell expression vector真核表达载体
aim:to construct an eukaryotic expression vector carrying human glutathione-s-transferase p1 gene (gstp1) and transfect it to a549 cells.
目的:构建人谷胱甘肽硫转移酶p1(gstp1)基因的真核表达载体,转染a549细胞,实现其真核表达。
aim: to construct eukaryotic expression vector carrying human antisense vegf gene and to study its effect on vegf expression and growth of renal cell carcinoma.
目的:构建反义vegf基因真核表达载体,研究其对肾癌细胞vegf表达的影响。
objective to construct the human g250 eukaryotic expression vector and establish the stable b16 cell line expressing human g250 in mice.
目的构建人g250真核表达载体,建立稳定表达人g250的小鼠黑色素瘤细胞系。
objective to clone the human angiotensin-converting enzyme 2 (ace2)and construct its eukaryotic expression plasmid.
目的克隆人血管紧张素转换酶2基因(ace2),并构建其真核表达载体。
objective to construct the eukaryotic expression vector of g2 gene of hantavirus envelope glycoprotein and to study its immunization effect.
目的构建汉坦病毒g2囊膜蛋白基因真核表达载体,进一步研究其免疫效果。
results rat il-10 eukaryotic expression vector had been constructed successfully, and had been transfected into rat chondrocyte.
目的构建大鼠il-10真核表达载体并在大鼠软骨细胞中进行表达。
objective to construct human il 16 eukaryotic expression vector, and analyse its function of inhibiting proliferation of tumor cells.
目的构建il-1 6真核表达载体,研究其对t淋巴瘤细胞的增殖抑制作用。
in this study, gene of envelope protein e2 was isolated from one hcv-infected person. then it was cloned and expressed in procaryotic and eukaryotic expression systems.
本文选取hcv包膜区e2基因作为研究对象,进行了基因克隆、蛋白表达以及蛋白的免疫原性和模拟表位的研究。
aim to clone human cd81 gene from peripheral blood lymphocytes, and construct its eukaryotic expression vector, and then express it in cos-7 cells.
目的从人外周血淋巴细胞中克隆出cd81基因,构建真核表达质粒,并在cos-7细胞中进行表达。
this research intended to construct eukaryotic expression vector with a site-directed mutation of porcine mstn propeptide gene, and verify its expression efficacy in c2c12 cells.
本研究旨在克隆通城猪含有第1个内含子的mstn前肽基因,构建真核定点诱变载体,并通过转染c2c12细胞验证载体表达的有效性。
introduction the construction and transfection of eukaryotic expression plasmid are commonly molecular biology method used in medical research.
序言真核表达质粒的构建和转染是目前医学研究中常用的分子生物学手段。
conclusion: a recombinant eukaryotic expression plasmid of amastin gene of leishmania donovani was successfully constructed, and can be expressed stably in the nih3t3 cells.
结论:成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒, 并且该基因在nih3t3细胞中获得了稳定表达。
objective:to construct an eukaryotic expression vector containing the open reading frame (orf) of long type of lrp16 gene and to detect its expression in hl60 cells.
目的:构建长型lrp16基因开放阅读框架序列的真核表达载体,并观察其在髓系白血病细胞系hl6 0细胞中的表达情况。
methods: anti vegf hairpin ribozyme gene eukaryotic expression vector was introduced into ovarian cancer skov3 cells by lipofectin mediation and positive clones were screened by g418;
方法:采用脂质体介导的方法将自行设计和构建的抗vegf发夹状核酶基因真核表达载体转染人卵巢癌skov3细胞,采用g418筛选获得阳性克隆;
objective:to establish nk cell receptor nkg2d eukaryotic expression vector and investigate on the cytotoxicity of nk cell line-yt transfected with nkg2d.
目的建立nk细胞受体nkg2d真核表达载体,通过转染nk细胞系yt,初步探讨nkg2d分子对yt细胞系杀伤功能的增强作用。
objective to construct an eukaryotic expression recombinant plasmid containing dense granule antigen 2(gra2) of toxoplasma gondii.
目的构建弓形虫致密颗粒蛋白gra2的真核表达重组质粒。
to clone mouse interleukin 4 (mil-4) truncated gene, construct its eukaryotic expression plasmid pfb-mil4 and express the truncated protein (murine il-4 receptor antagonist).
构建小鼠il-4截短型基因真核表达质粒,表达小鼠il-4受体拮抗体蛋白。
objective to analyse the in vitro expression of eukaryotic expression plasmid containing toxoplasma gondii dense granule protein gra8 gene.
目的:分析弓形虫致密颗粒蛋白gra8真核表达质粒的体外表达。
the recombinant eukaryotic expression plasmid was correctly constructed and can expressed in sp2/0 cell.
正确构建的复合表位真核表达质粒可在sp2/0细胞中表达;
conclusion construction of the eukaryotic expression vectors of wild type and mutant prpf31 genes is basic work for research on the mechanisms of retinitis pigmentosa caused by prpf31 mutation.
结论野生型和突变型prpf31基因真核表达载体的构建,为研究prpf31基因突变引起视网膜色素变性的机制奠定了基础。
conclusion eukaryotic expression vector of sirna targeting peg10 gene was successfully constructed, and should be a novel effective expression vector for hcc gene therapy.
结论本研究成功构建针对peg10的真核表达载体,可能成为肝癌基因治疗中的一种新型、高效的治疗载体。
results: antisense vegf gene eukaryotic expression vector was successfully constructed.
结果:成功构建反义vegf基因真核表达载体;
results restriction enzyme analysis and dna sequence analysis showed that pten gene was cloned and the eukaryotic expression vector was constructed successfully.
结果酶切和测序证实pten基因克隆和真核表达载体构建成功。
objective to construct the eukaryotic expression plasmids of hbv middle envelope protein and hbv e antigen.
目的构建乙型肝炎病毒(hbv)中片段表面抗原真核表达质粒和e抗原真核表达质粒。
objective to construct an eukaryotic expression vector carrying human glutathione-s-transferase(gst)a1gene and to provide study materials for toxicology and pharmacogenetic.
目的构建人谷胱甘肽硫转移酶a1真核表达系统,为毒理学、遗传药理学的研究等提供材料。
objective to clone the transporter associated with antigen processing 2 (tap2) gene and construct its eukaryotic expression vector.
目的克隆抗原处理相关转运体亚基tap2基因片段并构建真核表达载体。
objective to construct the eukaryotic expression vector which express the protein of vzv glycoprotein e in cos7 cells.
目的构建水痘带状疱疹病毒(vzv )糖蛋白e的真核表达载体并使其在cos7细胞中表达。
objective: to construct eukaryotic expression vector of human death receptor(dr5) and transfect ns-1 cells to establish stable ns-1 cell line.
目的:构建人死亡受体5(dr5)真核表达载体,转染ns-1细胞,建立稳定转染的ns-1细胞系。
to clone human bladder tumor e2f3 gene, construct its eukaryotic expression vector and expression in the biu-87 cell.
目的克隆人膀胱肿瘤组织e2f3基因及构建e2f3基因真核表达载体并研究其表达。