a method of using fluorescence microscopy to detect the presence of an antigen indirectly

objective:to evaluate the application of salt split skin indirect immunofluorescence (sss iif) in differential diagnosis of bullous pemphigoid (bp).

目的：探讨盐裂皮肤间接免疫荧光（iif）技术在大疱性类天疱疮（bp）鑒别诊断中的作用。

the results of indirect immunofluorescence and cell binding suggest that imms can effectively bind target cells while do not have nonspecific binding activity.

间接免疫荧光检测和imms与细胞的结合实验证明，所构建的imms可有效地和靶细胞结合。

antinuclear antibody (ana) was detected by indirect immunofluorescence experiments.

抗核抗体采用间接免疫荧光法检测；

firstly, we detected the distribution of cd155 on various human cell lines with indirect immunofluorescence staining and fcm analysis.

本实验首先采用间接免疫荧光染色和fcm分析了人多种细胞系的cd155表达。

methods t lymphoid subgroups and the percentage of nk cells were detected by the use of indirect immunofluorescence method the function of nk cells was detected by mtt.

方法采用间接免疫荧光法检测白血病患者t淋巴细胞亚群、nk细胞百分数，mtt法检测nk细胞杀伤活性。

method: indirect immunofluorescence labeling method.

方法：间接免疫荧光标记法。

the reactivity of human preformed natural antibodies with various porcine pancreatic cells was determined by indirect immunofluorescence staining technique .

采用间接免疫荧光染色法检测人预存天然抗体与猪胰腺细胞的反应性。

the serum fluorescence titres of anti-nuclear antibodies(ana) were tested by indirect immunofluorescence technique.

间接免疫荧光法检测血清中抗核抗体（ana）的荧光滴度。

methods anti-pr3 and anti-mpo antibody were determined by elisa and anca was determined by indirect immunofluorescence (iif) in 251 patients with systemic vasculitis and other autoimmune diseases.

方法对251例临床确诊为系统性血管炎和其他自身免疫性疾病患者血清，应用酶联免疫吸附试验（elisa）检测抗pr3抗体和抗mpo抗体；

with okt monoclonal antibodies, the t cell subsets in peripheral blood of 25 cases of liver cirrhosis and 30 normal controls were detected by indirect immunofluorescence technique.

应用okt 系列单克隆抗体，通过间接免疫荧光法，对25例肝硬化失代偿期患者及30例正常人外周血t细胞亚群进行检测。

indirect immunofluorescence was used to detect the hfrs antigen.

用间接免疫荧光方法检测hfrs抗原。

methods 3077sera from humans and 401 sera from rats were detected for antibodies of 14 different arbovirus species by indirect immunofluorescence assay(ifa)in 11 district of the south of china.

方法应用间接免疫荧光技术，对我国南方11个地区3077份人血清标本和40 1份鼠血清标本进行了14种虫媒病毒血清流行病学调查。

indirect immunofluorescence assay (ifa) was performed to identify the hanta viral antigen in rodent lung samples for the calculation of virus-carrying rates.

实验室采用间接免疫荧光法检测鼠肺汉坦病毒抗原，计算鼠带病毒率；

indirect immunofluorescence detection showed that positive reaction of diffuse cytoplasmic fluorescence was seen with antibody to keratin but not with antibodies to vimentin and desmin.

间接免疫荧光染色显示，小鼠早期胚胎细胞对角蛋白抗体呈阳性反应，对波形蛋白和结蛋白抗体呈阴性反应。

methods:the antiviral effect of drug was examined by an indirect immunofluorescence assay.

方法：运用间接免疫荧光法检测药物抗病毒作用。

the expression of exogenous genes were analyzed by indirect immunofluorescence assay (ifa).

采用间接免疫荧光法（ifa）检测外源基因的表达。

indirect immunofluorescence tests showed that the dna- and histone-depleted nuclei and chromosomes reacted positively with the anti-actin antibody.

免疫荧光实验结果指出，去除dna和组蛋白的细胞核和染色体与抗肌动蛋白抗体呈阳性反应。

t lymphocytes sub- sets were measured by indirect immunofluorescence technique.

用间接免疫荧光法检测t淋巴细胞亚群变化。

the ratio of cd/cd and the activity of nk, lak in 52 patients with hepatitis bwere studied adopting the methods of indirect immunofluorescence staining and mtt, respectively.

采用间接免疫荧光染色法、mtt法分别检测52例乙型病毒性肝炎（简称乙肝）患者cd／cd值、nk和lak细胞活性。

auto-antibodies were detected by indirect immunofluorescence test (iift).

自身抗体检测采用间接免疫荧光试验（iift）。

the transient expression of jev e in bhk 21 cells was detected by indirect immunofluorescence assay(ifa) with a monoclonal antibody 2h4 against jev e.

用其体外转染地鼠肾细胞bhk-21，经间接免疫荧光法（ifa）检测，证实了jev-e的瞬时表达。