melting curve assay and pcr microplate hybridization-elisa assay should be further improved to increase their sensitivity and specialty.
熔解曲线法和pcr微板核酸杂交法需要进一步完善以提高敏感性和特异性。
ymdd mutation of pcr melting curve and alt and ast and hbv-dna fluorimetry quantitative assay play an important role in the diagnosis and treatment of chronic hepatitis b.
pcr熔解曲线法测定ymdd变异株与alt和ast及hbv-dna荧光定量联合检测对慢性乙型肝炎患者的诊断,治疗有极其重要的价值。
with photoelectric test, digital keyboard input, and large-screen backlit lcd display, the apparatus may show the melting curve directly.
光电检测,数字键盘输入,大屏幕背光液晶显示,屏幕直接显示熔化曲线。
objective:to evaluate the feasibility and sensitivity of detecting the mutations of kras in colorectal carcinoma patients with high resolution melting curve analysis.
目的:探讨利用新的高分辨率熔解曲线分析法检测结直肠癌中kras基因突变的可行性。
the methods for detecting target bacteria by melting curve analysis were constructed.
对优化后的熔解曲线法进行特异性验证。
after pcr melting curve analysis was done.
pcr反应完毕作熔解曲线分析。
the hot pressing technological temperature on the processing of adhesive bonded fabric can be determined according to melting curve of fiber.
根据熔融曲线,可确定无纺布生产中的热粘合工艺温度在始熔至峰值间。
by choosing the appropriate lj potential parameter, the present approach can describe the melting curve of real molecules accurately.
通过选取合适的lj势参数,能很好地描述了真实分子的融化曲线。
conclusions:the new high resolution melting curve analysis is more efficient, more convenient than direct sequencing. moreover, it is a low-cost test, which is suitable for clinic test.
结论:新的高分辨率熔解曲线分析法在检测kras基因突变时比直接测序法具有耗时短,易操作的优点,并且检测成本低廉,适合在临床开展。