Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
目的:建立诺如病毒的荧光定量PCR检测方法,应用于急性胃肠炎的快速检测。
Results:Norovirus was successfully detected from epidemic sample of Lishui, the positive rates of real-time fluorescent quantitative PCR and ELISA were 78.26% and 55.43%, respectively.
结果:用实时荧光PCR方法成功的从丽水疫情样本中检测出诺如病毒,实时荧光PCR和ELISA的检出率分别为78.26%和55.43%。
To establish a TaqMan-based real-time fluorescent quantitative PCR assay for detection and quantitation of Hepatitis E virus.
目的建立灵敏、稳定、特异的实时荧光PCR方法,用于戊型肝炎病毒的定量检测。