aim:to construct a recombinant eukaryotic expression vector containing α-fetoprotein(afp) promoter-mediated hsv-tk suicide gene, and analyze its specific expression on afp-positive hepatoma cells.
目的:构建甲胎蛋白(afp)启动子介导的单纯疱疹病毒胸苷激酶(hsv-tk)重组真核表达载体,并检测其在afp阳性肝癌细胞株的特异性表达。
conclusion: a recombinant eukaryotic expression plasmid of amastin gene of leishmania donovani was successfully constructed, and can be expressed stably in the nih3t3 cells.
结论:成功地构建杜氏利什曼原虫无鞭毛体蛋白基因的真核表达重组质粒, 并且该基因在nih3t3细胞中获得了稳定表达。
objective to construct a new recombinant eukaryotic expression plasmid by fusing rattus ifn-γ-inducible protein-10 gene, and explore the expression of ip-10 in nih 3t3 cells.
目的构建大鼠干扰素诱导蛋白-10(ip-10)基因的真核表达质粒,并研究其在nih3t3细胞中的表达情况。
conlusion the human fhit recombinant eukaryotic expression plasmid was constructed successfully.
结论成功构建了重组人fhit真核表达质粒。