英汉例句

• methods we construct the rat antisense timp 1 recombinant plasmid which can be expressed in eucaryotic cells by rt nest pcr and the technique of recombinant dna.

  方法运用逆转录巢式（rtpcr）和重组dna技术，构建大鼠反义timp1真核细胞表达质粒，经脂质体包埋后，利用腹腔注射将其导入免疫性大鼠肝纤维化模型体内；

• methods the recombinant plasmid was transfected into nih/3t3 by calcium phosphate dna coprecipitation.

  方法重组质粒转染3t3细胞通过dna磷酸钙共沉淀法；

• the translocation of hmgb1 in thp-1 cells was observed under fluorescence microscope. results: the recombinant plasmid was verified by enzyme digestion.

  结果：重组质粒经酶切鑒定证明构建正确，并在thp-1细胞中大量表达。

• objective to construct recombinant plasmid with human atrial natriuretic peptide(anp)gene in fusion form for stable and high level expression of genetic engineering product anp in e. coli system.

  目的构建以融合蛋白形式在大肠桿菌中高效表达心钠素的重组质粒，稳定高效地获得基因工程产品心钠素。

• the recombinant plasmid was transformed into e.

  用化学法把重组质粒转化到e 。

• results:dna sequencing and pcr analysis indicated that the recombinant plasmid ppic9/bmp-7was constructed successfully and transformants were screened out for expression.

  结果：dna序列分析和pcr鑒定表明成功构建了人骨形成蛋白-7（bmp-7）毕赤酵母表达重组子。

• supercoiling is necessary for the expression of recombinant plasmid dna at transcription level.

  超螺旋化对于质粒dna在转录水平上的表达是必需的。

• after immunization, the recombinant plasmid pcdna-l1 was mainly distributed in the injection site and was removed as the time goes on.

  免疫后质粒主要分布在注射部位，且随着时间延长被清除；

• objective to construct recombinant plasmid for expression of chymopapain in e.

  目的构建木瓜凝乳蛋白酶的表达载体，并在大肠桿菌中表达。

• aim to construct a recombinant plasmid encoding toxoplasma gondii multi-antigen sag1/rop1 and to detect the immunity of the expressed product in e coli.

  目的构建含弓形虫主要表面抗原基因sag1和棒状体蛋白rop1复合基因重组质粒 ，并在大肠桿菌中表达 ，检测复合基因表达产物的免疫活性。

• fluorescence microscopy revealed green and relatively bright fluorescence in the sperm head after the recombinant plasmid was delivered into mice sperm at some ratio.

  ②将重组质粒按一定比例转染小鼠精子后，在荧光显微镜下观察到转染精子头部有较明亮的绿色荧光；

• aim: constructing a recombinant plasmid encoding gra1 protein of toxoplasma gondii and observing the protective immunity induced by recombinant plasmid dna vaccination.

  目的：构建弓形虫致密颗粒抗原-1（gra1）真核表达质粒，并观察重组质粒dna接种诱导的保护性免疫应答。

• by restriction enzyme identification, pcr and sequence analysis, the recombinant plasmid of the ha1 was successfully constructed.

  结果经双酶切、pcr及测序鑒定证实血凝素基因ha1区的真核表达载体构建成功。

• the strains with recombinant plasmid of hec-sod were induced and expressed as inclusion body.

  对含人ec-sod重组质粒的工程菌进行诱导表达形成包涵体。

• objective to construct a recombinant plasmid containing p30 gene of toxoplasma gondii and obtain recombinant protein methods p30 gene fragment was amplified from genomic dna of t.

  目的构建表达p30蛋白抗原表位的重组质粒及工程菌，获得纯化的p30蛋白抗原表位，用于检测弓形虫特异性抗体。

• the recombinant plasmid pet- 11d-ospc was identified with restriction endonuclease analysis and sequencing.

  采用酶切分析及序列测定等方法鑒定重组质粒的正确性。

• objective:1. recombinant plasmid pedf-gfp was transduced into the retina of the bn rats using cationic liposome, then observe the expression and location of the pedf-gfp.

  目的：1。将重组表达质粒pedf-gfp以脂质体介导的方法注射到bn大鼠视网膜下，观察目的基因能否表达及其定位。

• the recombinant plasmid was transformed into escherichia coli bl21 (de3) and was then identified by pcr, double enzyme digesting and sequencing.

  转化大肠埃希菌bl21（de3），酶切、pcr及测序鑒定其插入序列的正确性。

• objective:to construct the recombinant plasmid of hpv16l1-e7c and study its cellular immunity.

  目的：构建hpv16l1-e7c重组质粒并研究其细胞免疫性。

• the supernant of hcc cells transduced with the recombinant plasmid can support the growth of tf 1 cells.

  表达产物能分泌到肝癌细胞外，分泌到细胞外的产物用tf-1细胞检测证明具有生物学活性。

• the recombinant plasmid was confirmed by digestion and sequence, then transformed into e. coli bl21.

  重组质粒经酶切和测序证实正确，然后转化大肠桿菌bl21。

• objective to select an optimal combined set of carrier, introducing route and dose of recombinant plasmid of augmenter of liver regeneration(alr) for alr gene therapy.

  目的筛选肝再生增强因子重组质粒基因治疗时较优的载体、途径和剂量及三者的最佳组合。

• the present invention also discloses the nucleotide sequence encoding the recombinant streptococcus pneumoniae resisting polypeptide and recombinant plasmid containing said nucleotide sequence.

  本发明还公开了编码该重组抗肺炎链球菌多肽的核苷酸序列，以及含有所述核苷酸序列的重组质粒。

• was identified by pcr and the recombinant plasmid correct size, and insert a green fluorescent protein, methods and proved to insert the right direction.

  并经酶切鑒定与pcr方法证明重组质粒大小正确，且插入了绿色荧光蛋白，并证明插入方向正确。

• objective to construct an eukaryotic expression recombinant plasmid containing dense granule antigen 2(gra2) of toxoplasma gondii.

  目的构建弓形虫致密颗粒蛋白gra2的真核表达重组质粒。