It explained that reformed CTAB method could extract the genomic DNA of Pyrus successfully, and the optimum RAPD reaction system could amplificate RAPD in Pyrus L…
说明改良的CTAB法能成功用于梨属植物DNA提取,建立的最佳反应体系可用于梨属植物RAPD扩增。
Conclusion: The modified technique based on CTAB method may be efficient and reliable in isolating high quality DNA for AFLP analysis from medicine plants rich in polyphenols and amylose.
结论该技术可有效地从富含多酚和多糖的药用植物组织中分离出高质量dna。
Method:Two different methods, conventional CTAB method and modified CTAB method, were applied to extract genomic DNA from leaves of Lonicera japonica Thunb.
方法:采用改良CTAB法从忍冬叶片中提取DNA,通过琼脂糖凝胶电泳、紫外分光光度法、酶切检测其质量,并与常规CTAB法比较。
The results showed that the DNA purified by the modified CTAB method was suitable for PCR and other molecular analysis, and the qualitative PCR was an effective way to screen the GMO.
实验结果表明,用改良的CTAB法提纯得到的大豆加工产品中的DNA完全可以满足PCR等分子生物学分析,而且定性PCR法能有效检测大豆及其加工产品中转基因成分。
But the total RNA extracted from CTAB method had lower yield and purity. Therefore, improved CTAB method could extract high quality total RNA of banana leaves.
而CTAB法提取的总rna产量较低,且纯度不高。说明改良CTAB法能有效从富含多酚和多糖的香蕉叶片中提取高质量的总rna。
Conclusion: The improved CTAB method could be used for isolating total DNA with high quality from guava leaves.
结论:传统CTAB法经过改良,可用于快速提取番石榴高质量dna。
In this experiment, both the modified CTAB method and the boiling method were used to extract the maize total genome DNA.
本试验用改良的CTAB法和煮沸法两种方法提取玉米基因组dna。