2025-10-31 20:08 浏览次数 9
免疫小鼠
the recombination fusion protein was purified by lysis, washing, denaturation and renaturation. the specific band of expression was excised from the gel and used to immunize mice .
通过裂解、洗涤、变性、复性等方法对包涵体蛋白进行处理,以电泳切胶回收所获得的纯化产物作为抗原,免疫小鼠制备抗重组蛋白血清。
using the protease as antigen to immunize mice and the specific monoclonal antibodies were produced.
以纯化蛋白免疫小鼠,制备特异性单克隆抗体,对纯化后的人多抗和鼠单抗进行鑒定。