dual linear gradient elution can be performed with two specially made glass container and a high pressure constant flow pump.
用两个特制的玻璃容器及一台平流泵即可实现二元线性梯度洗提。
method: rp-hplc, linear gradient elution and lc/ms, etc. were used to optimize the fingerprint determination method, and identify the main peaks in the hplc fingerprint.
方法:利用rphplc、线性梯度洗脱筛选最佳测试条件,并应用lc/ms技术指认指纹图谱中的主要色谱峰。
objective: to develop a hplc linear gradient elution method for rapid separation of the components of bleomycin, and for determination of bleomycin a2 and bleomycin b2. method;
目的:研究应用反相离子对色谱梯度洗脱法以较快速度分离硫酸博莱霉素组分,并尝试梯度洗脱条件下测定其有效成分a_2、b_2的含量。