optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in e.
对大肠桿菌表达的重组人胰岛素原包涵体蛋白的变性复性条件进行了优化。
the renaturation of polyphenol oxidase from tobacco denatured in 6 0 mol/l guanidine hydrochloride(gdnhcl) was investigated.
研究了盐酸胍变性烟草多酚氧化酶后的复性。
aim: to study the application of urea gradient size exclusion chromatography (sec) in the renaturation with simultaneous purification of recombinant human stem cell factor (rhscf) expressed in e.
目的:用脲梯度体积排阻色谱(sec)复性并同时纯化在大肠桿菌中高表达的人干细胞因子。
the conditions of purification, denaturation and renaturation of recombinant bovine growth hormone(bgh) were researched in order to get a good renaturation result.
为了取得较好的复性效果,对重组牛生长激素的纯化及变复性条件进行了研究。
by denaturation find renaturation ac vitro, 300000 units of active pro-urokinase were obtained from 1 l culture medium.
经体外交复性,从1升培养基中可获得300000单位的活性尿激酶原。
the kinetics of the renaturation and reactivation of glucoamylase denatured by guanidine hcl was determined by means of the uv differential absorbance measurement and fluorescence emission spectra.
本文利用荧光、紫外差光谱研究了根霉葡萄糖淀粉酶在盐酸胍变性后的复性、复活动力学。
after isolation of the inclusion bodies, the optimum conditions of denaturation and renaturation were studied.
包涵体经分离洗涤后,探索了对其变性复性的最佳条件。
optimization of mobile phase composition in pflc for protein renaturation with simultaneous purification is thus much more important than that in usual liquid chro.
蛋白折叠液相色谱法(pflc)用于变性蛋白质复性并同时纯化时对流动相组成及其洗脱条件的要求远较通常的液相色谱法高。
objective to examine the changes of conformation and activity of human placental alkaline phosphatase(hpap) in denaturation and renaturation of guanidine chloride, and to study its folding mechanism.
目的观测人胎盘堿性磷酸酶(hpap)在盐酸胍变性和复性过程中构象与活力的变化,探讨其折叠机制。
the recombinant protein of purification and renaturation may be a potential candidate to be a serodiagnostic reagent.
结论具有天然结构和高纯度的重组融合蛋白有可能成为有效的结核病血清学诊断的候选抗原。
inclusion body protein renaturation is a important procedure in the biotechnology.
包涵体蛋白的复性是生物工程下游技术中的一个重要难题。
after purification, the target protein further renaturation 2 - bmp via degeneration dissolving, load dialysis bag.
纯化后的的目的蛋白进一步复性,bmp-2经变性液溶解,装入透析袋内。
the renaturation of gene recombinant protein is the most important technique in the field of biotechnology which's many methods have been established.
基因重组蛋白质的复性是当前生物工程领域的重要研究课题。
the process of renaturation is established in the understanding to the mechanism of protein folding in vitro.
变复性实验是建立在对蛋白质体外折叠机制的了解的基础上。
a moderately repetitive dna sequence from rice had been cloned by the method of dna renaturation kinetics.
大量重复dna顺序的存在是高等植物基因组的重要特征之一。