英汉例句

• methods:by using rt pcr method, the expression of hematopoietic growth factor in placenta was assessed.

  方法：通过rt- pcr方法检测胎盘组织中造血相关因子的表达情况。

• rt pcr assay showed induced cells expressed osteocalcin.

  pcr显示诱导细胞表达成骨细胞特异的骨钙素。

• methods the cell cycle and the expression of related genes were analyzed by the methods of immunocytochemistry, protein blotting, rt pcr and flow cytometry.

  方法利用免疫细胞化学，蛋白质印迹法，rt- pcr和流式细胞术等方法分析细胞周期和对与细胞周期相关的基因表达进行研究。

• the rna expression level of leptin receptor was examined by rt pcr .

  应用免疫沉淀法检测瘦素受体的酪氨酸磷酸化程度。

• the expression of e7 gene was examined by rt pcr and the indirect immunofluorescence.

  e7基因表达的检测采用rt pcr和间接免疫荧光实验。

• conclusiondue to specificity on enterovirus, the rt pcr method has some value in the diagnosis of enterovirus infections.

  结论该rt-pcr方法具有肠道病毒特异性，在肠道病毒感染的诊断中有一定应用价值。

• methods of elisa, nonradioactive molecular hybridization and rt pcr were applied in the detection of rice grassy stunt virus (rgsv).

  将间接elisa、非放射性分子杂交和rt pcr三种方法应用于水稻草矮病毒（rgsv）的检测。

• methods: the expression of b7 in 22 cases of renal cell carcinoma was studied by rt pcr and immunochemical methods.

  方法：本文应用rt?pcr和免疫组织化学技术检测了22例肾细胞癌组织b7的表达。

• methods p gp and mdr1 mrna levels were measured by immunohistochemistry abc and rt pcr assay in ovarian carcinoma.

  方法应用免疫组织化学（abc） 法及改进的逆转录聚合酶链反应（rtpcr）分别检测pgp和mdr1 基因mrna 的表达水平。

• methods the total rna were prepared from z10 virus infected cells and the rt pcr product was cloned into t vector, sequenced and analyzed using dnastar software.

  方法从z10株病毒感染的细胞提取总rna ，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。

• method expression of cgrp mrna in lymphocytes was determined by the semi quantity rt pcr and the cgrp levels in plasma were measured by ria.

  方法采用逆转录聚合酶链反应（rt-pcr）测定大鼠淋巴细胞cgrpm rna水平。用放射性标记法测定血浆cgrp水平。

• both rt pcr and fish techniques were proved to be useful for detecting tel aml1 fusion gene and suitable for clinical diagnosis and prognosis evaluation.

  pcr和fish技术是检测telaml1的有效方法，并为临床诊断和预后估计提供了有一定价值的手段。

• methods rt pcr products were amplified from s85 46 virus infected cells, cloned into t vector, sequenced and analyzed using dnastar software.

  方法将特异性引物从s8546病毒感染细胞pcr扩增的产物克隆于t载体，正确的克隆纯化后测序，应用dnastar软件比较分析。

• quantitative rt pcr method were used to detect the mrna levels of multidrug resistance gene in 32 leukemia children.

  应用逆转录pcr结合同位素定量分析，对32例儿童白血病患者的多源耐药基因表达水平进行了研究。

• addition of exogenous insulin to the culture decreased the insulin mrna expression remarkably on rt pcr when dexamethasone increased the insulin gene expression at the transcriptional level.

  rt pcr也证实在转录水平上外源性胰岛素明显抑制胰岛素基因的表达，而地塞米松上调其表达。

• methods the genes encoding antibody variable regions were cloned by rt pcr from hybridoma cells and expressed on bacterial phage surface.

  方法利用rtpcr 方法扩增抗体可变区基因并表达于噬菌体表面。

• methods the total rna were prepared from z10 virus infected cells and the rt pcr products was cloned into t vector, sequenced and analyzed by using dnastar software.

  方法从z10病毒感染的细胞提取总rna ，将逆转录聚合酶链反应（rt pcr）扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas tar软件分析比较。