Methods: The improved cultivating technique of human keratinocyte with serum was used.
方法:采用改良的有血清培养技术进行人体角朊细胞的培养。
Selection and identification of human epidermal stem cells in vitro: To select human keratinocyte stem cells in vitro.
人表皮干细胞体外筛选及鑒定的实验研究:探讨体外筛选及鑒定表皮干细胞的方法。
Objective to understand the effect of products secreted by dermatophyte and candia albican on human keratinocyte in vitro.
目的了解表皮癣菌和白念珠菌培养产物对体外培养人角质形成细胞的作用。
Conclusion: The improved methods of manual purification, cool digestion and dermal scratching may increase the human keratinocyte livability and growth speed.
结论:采用人工纯化法、冷消化法和真皮刮除法等改良方法可提高体外培养的人表皮角质细胞存活率及生长速度。
Objective to explore the cultivating technique of human keratinocyte under laboratory conditions, and to provide reliable cells resource for the experimental study on keratinocyte in many ways.
目的:探讨实验室条件下人体角质形成细胞的培养技术,为角质形成细胞的多方面研究提供可靠的细胞来源。
Method: Use tissue engineering technology to separate and culture human keratinocyte in vitro and observe them using MTT method and histological and electron microscopic methods.
方法:应用人表皮细胞的分离、培养技术,并进行MTT检测、病理切片以及电镜检测等手段进行观察。