aim to establish a cell line chl udp glucuronosyltransferase gene (udpgt1a9) which will stably express human udpgt1a9 protein and determine the activity of expressed udpgt1a9 in drug glucuronidation.
conclusion: we constructed an frt cell line that could stably express ut-a2 in plasma membrane in the non-renal epithelia cell. the cell line will be used to screen ut-a2 inhibitors.