a crude antibacterial preparation(cap) was obtained by adding ammonium sulphate to supernatant fluid of b. subtilis to a finial saturation of 60%.
于培养液上清中加入60%饱和度的硫酸铵沉淀可得蛋白粗提液。
the content of mda in the supernatant fluid of cultured ec cells was detected by spectrophotometry.
分光光度计测定细胞上清液中丙二醛的含量。
the culture supernatant fluid was collected, the mass concentrations of il-8 and et-1 were measured by elisa, and the concentration of nitric oxide(no) was determined by nitrate reduction method.