conclusion the recombinant human folr-1 gene clone has been established successfully and thus provides a basis for further research of the immunotherapy or targeting therapy for ovarian carcinoma.
method:the mutation and deletion of exon5, exon6 and exon8 of pten gene were detected by pcr, gene clone and sequencing in mucoepidermoid carcinoma of parotid gland and normal gland tissue.
cultivating valuable kiwifruit cultivars still be a great challenge, gene clone and molecular markers are widely applied in breeding of kiwifruit, and reach some achievements.