based on the homologous recombination, the plasmid prg of reverse gyrase gene knock-out was constructed and transformed into the sulfolobus islandicus and the single crossover mutant was isolated.
重组质粒反复转化冰岛硫化叶菌,得到质粒整合到宿主染色体上的单交换菌株,但没有获得反螺旋酶基因缺失的双交换转化子。