gradient centrifugation
[生化] 梯度离心
2026-05-06 09:33 浏览次数 17
[生化] 梯度离心
density gradient equilibrium centrifugation密度梯度平衡离心
gradient t centrifugation梯度离心分离法
Percoll gradient centrifugationPercoll梯度离心法
density gradient zonal centrifugation密度梯度区带离心
isopycnic density gradient zonal centrifugation等密度梯度区带离心法
preformed gradient isodensity centrifugation预成梯度等密度离心
Pass gradient pad centrifugation过垫梯度离心
gradient density centrifugation梯度密度离心
percoll discontinuous gradient centrifugationpercoll不连续梯度离心
the traditional sucrose gradient centrifugation for protein initiation translation analysis was improved greatly.
本实验全面改进了用于蛋白质起始翻译阶段研究的传统介质梯度离心法。
methods: bone marrow was obtained from healthy new zealand rabbits, by using density gradient centrifugation bmscs were isolated and were then cultured in vitro.
方法:取健康新西兰幼兔骨髓,密度梯度离心法分离骨髓间充质干细胞并进行体外扩增培养、成骨诱导分化及鑒定。
conclusion:the purify and amount of extravillous cytotrophoblast cells derived from improved compound enzyme digestion gradient centrifugation method increase significantly.
结论:该实验室改良的复合酶消化梯度离心法获得绒毛外细胞滋养细胞纯度和数量显着提高。
conclusion density gradient centrifugation combined with adherence cells filtration is an effective way to obtain epc derived from mice spleen.
结论通过密度梯度离心联合贴壁筛选可以获得脾脏epc。
resultsthe rat bone marrow mononuclear cells collected by density gradient centrifugation can differentiate into endothelial progenitor cells with the induction of the egm-2 culture.
结果对采用密度梯度离心法获取的大鼠骨髓单核细胞在体外进行egm-2诱导培养,可分化为内皮祖细胞。
methods the method of sucrose gradient centrifugation was used to purify human herpesvirus-6b(hhv-6b);
方法采用蔗糖密度梯度离心法进行病毒纯化;
the purity of human embryonic msc which isolated by density gradient centrifugation and cultured by mem culture medium in vitro is high. the activity of msc is good.
用密度梯度离心法分离后mem培养基体外培养的人胚胎msc纯度高,生物活性好。
methods human fetal bone marrow was obtained and gradient centrifugation performed to isolate cell pellets of low density that contained mscs followed by cell culture.
方法抽取流产胎儿股骨骨髓,梯度离心,取含有骨髓间充质干细胞的低密度血小板层进行培养;
methods: autologous bmscs were isolated by density gradient centrifugation with stem cell extract liquid to prepare stem cell suspension, and were intramuscularly injected into the suffered limbs.
方法:实验采用干细胞分离液,利用梯度密度法分离患者自体骨髓干细胞,制备干细胞悬液。
recombinant adenoviruses were generated by packaging of hek293 and purified by velocity density gradient centrifugation in caesium chloride solutions.
腺病毒载体经hek293细胞包装产生含反义片段的重组腺病毒,用氯化铯密度梯度离心法获得纯化的高滴度腺病毒;
coal maceral concentrates of high purity were prepared from four different rank coals by density gradient centrifugation method.
经等密度梯度离心分离,从褐煤、长焰煤、气煤和贫煤四种不同变质程度煤中获得了高纯度的有机显微组分。
the titer was assayed and csc1 density gradient centrifugation and purification were applied.
测滴度并用氯化铯梯度离心纯化备用。
objective to compare the reliability of immunomagnetic beads and density gradient centrifugation in purification and isolation of neutrophilic granulocytes from human peripheral blood.
目的比较免疫磁珠法和密度梯度离心法分离纯化人外周血中性粒细胞的可靠性。
conclusion the method of mechanically, gradient centrifugation can successfully separate the tdc. gm-csf and scf can promote the growth of the tdc and provide satisfactory growth conditions for them.
结论梯度离心法能成功分离胸腺树突状前体细胞,gmcsf和scf体外诱导可提供胸腺树突状细胞原代培养较为满意的生长条件。
as technological research findings will be industrialization, we choose the sucrose density gradient centrifugation as purified method and study the postorder work of purified virus-containing fluids.
鑒于产品开发要求,试验研究确定选用蔗糖密度梯度离心方法作为流感疫苗的纯化手段。
results: sucrose density gradient centrifugation gave iscoms as a white sediment in the centrifuge tube.
结果:浓缩样品液经蔗糖梯度超速离心后,在离心管底部可见白色病毒带;
methods: bmscs were obtained from long bone of zelanian rabbits aged 4-8 weeks, the cells were isolated by gradient centrifugation and purified by anchoring culture.
方法从4~8周龄的新西兰大白兔的左右两侧股骨大转子部抽取骨髓,应用密度梯度离心法和贴壁培养法进行骨髓基质干细胞的分离和纯化。
methods: bmscs were harvested from rats by density gradient centrifugation and adherent culture.
方法:以密度梯度离心与贴壁培养法获得大鼠骨髓间充质干细胞。
separationby gradient centrifugation increased cell trauma and could not separate the different cells.
梯度离心的分离方法增加细胞损伤,不能达到分离不同细胞的效果。