英语释义

• a hybrid cell resulting from the fusion of a lymphocyte and a tumor cell; used to culture a specific monoclonal antibody

片语

hybrid cell[细胞] 杂交细胞；[组织] 杂种细胞

hybridoma technic杂交瘤技术

hybridoma technologyHybridoma technology is a technology of forming hybrid cell lines (called hybridomas) by fusing a specific antibody-producing B cell with a myeloma (B cell cancer) cell that is selected for its ability to grow in tissue culture and for an absence of antibody chain synthesi The antibodies produced by the hybridoma are all of a single specificity and are therefore monoclonal antibodies (in contrast to polyclonal antibodies).

hybridoma culture杂交细胞培养

hybridoma cell杂交瘤细胞

humanhuman hybridoma人

hybridoma producing杂交瘤细胞株

lymphocyte hybridoma淋巴细胞杂交瘤

hybridoma product杂交瘤生成

hybridoma a杂交瘤

英汉例句

• Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.

  方法利用RT-PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。

• Absorption of human-human hybridoma cells can abolish the human hybridoma growth factor (HGF) activity but not the BCGF activity.

  但人-人B细胞杂交瘤可吸收掉上述BCGF活性上清中的促杂交瘤细胞生长因子(HGF)活性，而对BCGF活性无明显影响。

• We have obtained the second group of monoclonal antibodies against human platelet by means of hybridoma technique.

  用杂交瘤技术获得了第二组抗人血小板的单克隆抗体。

• Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.

  方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鑒定。

• Methods The genes encoding antibody variable regions were cloned by RT PCR from hybridoma cells and expressed on bacterial phage surface.

  方法利用RTPCR方法扩增抗体可变区基因并表达于噬菌体表面。

• Highly positive rate was obtained. The four hybridoma cells were separately cloned nine times in proper sequence and can continuously secrete high-titer McAbs.

  它们分别经过九次有限稀释连续培养九个月仍能持续地分泌高滴度抗体，并且抗体分泌量随克隆化次数的增多而增加。

• Electrical parameters affecting gene transfer, such as the field strength ( Voltage ), pulse durations and pulse frequency ect. and optimum transfer conditions in murine hybridoma cells were studied.

  研究了电场强度、脉沖宽度、脉沖次数等电场参数对基因转化频率的影响和小鼠杂交瘤细胞最适电转移条件。

• Then the hybridoma cell lines secreting high titer of McAbs were screened.

  目的建立分泌抗华支睪吸虫代谢抗原的单克隆抗体杂交瘤细胞株。

• This is a reliable method to select the hybridoma cells for McAb of IBDV.

  是研究筛选抗ibd V单抗杂交瘤细胞的可靠方法。

• But it is difficult to make humanized monoclonal antibody with hybridoma technique. Antibody library technique coming forth in the early 90s provides the new method to get the antibody.

  采用杂交瘤技术制备人源性单克隆抗体存在许多困难，90年代初期出现了抗体库技术，为抗体的制备提供了新的途径。

• Objective: To obtain hybridoma cell line which can secrete anti-gonyautoxin2,3 (GTX2,3) monoclonal antibodies(mAb) and analysis the character of the mAb.

  目的：建立抗麻痹性贝毒素GTX2，3单克隆抗体杂交瘤细胞系，制备抗GTX2，3单克隆抗体，并鑒定其免疫学特性。

• Methods The titer and affinity of anti-A secreted by hybridoma were tested by tube method and slide method.

  通过试管法和玻片法检测抗a抗体的效价和亲和力。

• The chromosome of the positive hybridoma and the character of the mAb were analysed.

  对所得阳性杂交瘤细胞作染色体分析并对单克隆抗体进行特性分析。

• At last, we got one positive hybridoma cells named 4d11.

  筛选共得到一株阳性杂交瘤细胞，命名为4d11。

• Since 1975 when the technology of hybridoma and monoclonal antibody was developed, many investigators have been paying close attention to antibody targeting therapy for patients with cancer.

  自从1975年发现了单克隆抗体技术和不久后提出生物导弹设想以来，导向治疗受到许多科研工作者的关注。

• Objective To determine the effect of diffusion chamber for immune isolation and the proliferation of allogenic hybridoma within diffusion chamber in mice.

  目的观察自制细胞扩散匣的免疫隔离作用及匣内异基因瘤细胞的在体生长情况。

• The principles of hybridoma technique are involved in some advantages, high specificity, sensitivity, convenience and quickness.

  杂交瘤技术原理和操作过程包含了其许多优点，特异性强、方便、敏感和快速。

• It suggests that the hybridoma has a stronger secretory ability.

  杂种细胞的分泌能力较强。

• Methods Hybridoma cell line excreting monoclonal antibody against total aflatoxins was produced by using B cell hybridoma technique.

  方法利用B细胞杂交瘤技术，建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。

• The splenocytes of immunized BALB/c strain mice with purified Ig were fused with myeloma cell line, NS-1 . The supernatants of hybridoma were screened by ELISA.

  将纯化之球蛋白片段免疫 BALB/c小鼠后， 取脾脏细胞与小鼠骨髓瘤细胞株 NS-1 进行细胞融合。

• To generate the recombinant antibodies, the key point is the cloning and the sequencing of the VH and VL genes from antibody secreting hybridoma cell line.

  而要研制基因工程抗体，鼠源性抗体可变区基因的克隆与测序是关键。