To generate the recombinant antibodies, the key point is the cloning and the sequencing of the VH and VL genes from antibody secreting hybridoma cell line.
而要研制基因工程抗体,鼠源性抗体可变区基因的克隆与测序是关键。
Then the hybridoma cell lines secreting high titer of McAbs were screened.
目的建立分泌抗华支睪吸虫代谢抗原的单克隆抗体杂交瘤细胞株。
Objective: To obtain hybridoma cell line which can secrete anti-gonyautoxin2,3 (GTX2,3) monoclonal antibodies(mAb) and analysis the character of the mAb.
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.