objective:to observe the light electron microscopic structure and the immunohistochemical characters of the skeletal muscle satellite cells in vitro in the myogenesis of the neonatal mice.
目的:观察体外培养的小鼠骨骼肌卫星细胞在成肌过程中的光电镜结构及免疫组织化学特性。
skeletal muscle satellite cells are quiescent mononucleated myogenic cells, located between the sarcolemma and basement membrane of terminally-differentiated muscle fibres.
骨骼肌卫星细胞是骨骼肌中位于肌细胞膜和基膜之间的具有增殖分化潜力的肌源性细胞。
establishing the simple and fast method to identify and filter skeletal muscle satellite cells has the practical meaning for culture and research and application of skeletal muscle satellite cells.
建立简便快速的鑒定和筛选骨骼肌卫星细胞的方法对于卫星细胞培养、研究和应用都具有实际的意义。
rat triceps brachii muscle was acquired to separate skeletal muscle satellite cells with the two-step method of collegenase-1 and trypsin and were cultured and subcultured in vitro.
取大鼠肱三头肌,采用二步消化法分离骨骼肌卫星细胞,体外进行原代培养和传代培养。
in this study, a method for culture and identification of the porcine skeletal muscle satellite cells was established and improved.
本研究中,我们建立并完善了猪骨骼肌卫星细胞的培养方法。