methods:1. primary rat skeletal muscle satellite cell was cultivated in vitro. the cultured cells were identified with cellular immunochemical stain.
方法:1)体外分离培养大鼠骨骼肌卫星细胞,免疫组化鑒定骨骼肌卫星细胞并检测其纯度。
objective: to observe the effects of transplanted skeletal muscle satellite cell on denervated skeletal muscle atrophy in vivo.
目的:观察骨骼肌卫星细胞种植对在体失神经骨骼肌萎缩的影响。
the auto- skeletal muscle satellite cell cultured in vitro for 2 weeks were transplanted into the myocardial region of the rats in the transplantation group.
将体外培养2周的大鼠自体卫星细胞以注射的方式移植到移植组大鼠梗死区周围。